Figure 1
A GA n microsatellite in the hFli1 promoter modulates activity in a human T cell line. A. Construct showing the location of distal and proximal promoters, transcription start sites (arrows) and GA microsatellite (white box) in the Fli1 promoter. Numbering is relative to the +1 translation start site. B. Sequence of the human -502/-37 region is aligned with the equivalent -505/-37 region from mouse. The GA microsatellite is in bold and primer sites used to amplify the microsatellite-containing region in human genomic DNA samples are underlined. C. The -502 to -37 region of the human Fli1 promoter shown in B with different lengths of the GAn microsatellite was cloned from genomic DNA samples of unaffected controls in the CLU study into the pGL3 Basic reporter vector. Constructs were transfected into Jurkat T cells and assayed for promoter activity. Expression is presented relative to the pGL3 Basic empty vector, which was set to 1. Results are an average of three independent transfections performed with two independently derived clones. *P < 0.005.