Figure 4

Identification of the antinuclear antibodies in sera of C57BL/6J mice. Representative patterns of cellular staining of HEp-2 cells by sera diluted at 1:40 prepared from sera taken from C57BL/6 mice cannulated with Ad5-LacZ or Ad5-IL17A vectors at 7 wks of age with pre-treated mice (baseline) at 6 wks of age (n = 4) (a-c), and cannulated at 16 wks of age with Ad5-LacZ or Ad5-IL17A and pre-treated mice (baseline) at 15 wks of age (n = 4) (d-f) with negative control using secondary antibody only (g) and positive control with standard nuclear speckled serum (h). Representative patterns were determined with n = 4 for two baselines and n = 7 for each time point presented in the figure. Fixed HEp-2 substrate slides were incubated with individual mouse sera diluted 1:40, 1:80 and 1:160 followed by development with FITC-conjugated goat anti-mouse IgG. Fluorescent patterns were detected by fluorescence microscopy at 400X magnification.