Figure 2

MIG/CXCL9 and IP-10/CXCL10 are highly expressed, but CXCR3 is decreased, in SSc and normal skin. Frozen sections of proximal and distal SSc and normal skin were stained for MIG/CXCL9, IP-10/CXCL10, or CXCR3. (A), MIG/CXCL9 was highly expressed in the stratum spinosum of normal (48%), proximal SSc (52%), and distal SSc (57%) skin. (B), IP-10/CXCL10 was uniformly expressed in the stratum basalis of normal, proximal SSc, and distal SSc skin at 100%. (C), CXCR3 was significantly decreased on ECs in proximal SSc (25%) and distal SSc skin (21%) compared to normal skin (54%). Representative photos of CXCR3 immunohistological staining in normal (D) and distal SSc skin (E) are shown. Arrows indicate positive staining. Means are given with the SEM. n = the number of patients. P < 0.05 was considered significant.