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Figure 8 | Arthritis Research & Therapy

Figure 8

From: Modulation of transforming growth factor beta signalling pathway genes by transforming growth factor beta in human osteoarthritic chondrocytes: involvement of Sp1 in both early and late response cells to transforming growth factor beta

Figure 8

Gene expression pattern induced by transforming growth factor beta 1 under Sp1 ectopic expression. Human articular chondrocytes (HAC) were nucleofected with pEVR2 or pEVR2-Sp1. Thereafter, the media were replaced with DMEM + 2% FCS for 24 hours before adding transforming growth factor beta 1 (TGFβ1) (5 ng/ml) for an additional 24 hours of incubation. Total RNA was then extracted and real-time RT-PCR analysis was performed. Histograms represent the relative COL1A1, COL2A1, COL10A1, aggrecan or Sox9 mRNA levels versus GAPDH.

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