Reduced immunomodulation potential of bone marrow-derived mesenchymal stem cells induced CCR4+CCR6+Th/Treg cell subset imbalance in ankylosing spondylitis

Table 2 Antibodies used to detect CCR4⁺CCR6⁺ Th and Treg cells in PBMCs and phenotype BMSCs by flow cytometry

Antibody	Isotype	Clone/fluorochrome	Concentration (μg/ml)	Source
CD105 (ENG)^a	Mouse IgG₁	266/FITC	10	Becton Dickinson (Bedford, MA, USA)
CD73 (NT5E)^a	Mouse IgG₁	AD2/FITC	20	Becton Dickinson (Bedford, MA, USA)
CD90 (THY1)^a	Mouse IgG₁	5E10/FITC	1	Becton Dickinson (Bedford, MA, USA)
CD34^a	Mouse IgG₁	581/FITC	50	Southern Biotech (Birmingham, AL, USA)
CD45 (PTPRC)^a	Mouse IgG₁	HI30/FITC	10	Caltag (Burlingame, CA, USA)
CD14^a	Mouse IgG₁	61D3/PE	200	Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA)
HLA-DR^a	Mouse IgG_2a	L243/FITC	200	Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA)
CD4^b	Mouse IgG_2b	OKT4/FITC	12	eBioscience (San Diego, CA, USA)
CD25^b	Mouse IgG_1,κ	BC96/APC	25	eBioscience (San Diego, CA, USA)
FOX-P3^b	Mouse IgG₁	236A/E7/PE	50	eBioscience (San Diego, CA, USA)
CCR4^b	Mouse IgG_1,κ	1G1/PE-Cy7	200	BD Pharmingen™ (Basel, Switzerland)
CCR6 (CD196)^b	Mouse IgG_1,κ	11A9/PE	200	BD Pharmingen™ (Basel, Switzerland)

^aAntibodies used to phenotype bone marrow-derived mensenchymal stem cells (BMSCs) by flow cytometry. ^bAntibodies used to detect CCR4⁺CCR6⁺ CD4⁺ T-helper (Th) cells and forkhead box P3-positive regulatory T (Treg) cells by flow cytometry. PBMC, peripheral blood mononuclear cell.

ISSN: 1478-6362