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Table 2 Antibodies used to detect CCR4+CCR6+ Th and Treg cells in PBMCs and phenotype BMSCs by flow cytometry

From: Reduced immunomodulation potential of bone marrow-derived mesenchymal stem cells induced CCR4+CCR6+Th/Treg cell subset imbalance in ankylosing spondylitis

Antibody Isotype Clone/fluorochrome Concentration (μg/ml) Source
CD105 (ENG)a Mouse IgG1 266/FITC 10 Becton Dickinson (Bedford, MA, USA)
CD73 (NT5E)a Mouse IgG1 AD2/FITC 20 Becton Dickinson (Bedford, MA, USA)
CD90 (THY1)a Mouse IgG1 5E10/FITC 1 Becton Dickinson (Bedford, MA, USA)
CD34a Mouse IgG1 581/FITC 50 Southern Biotech (Birmingham, AL, USA)
CD45 (PTPRC)a Mouse IgG1 HI30/FITC 10 Caltag (Burlingame, CA, USA)
CD14a Mouse IgG1 61D3/PE 200 Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA)
HLA-DRa Mouse IgG2a L243/FITC 200 Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA)
CD4b Mouse IgG2b OKT4/FITC 12 eBioscience (San Diego, CA, USA)
CD25b Mouse IgG1,κ BC96/APC 25 eBioscience (San Diego, CA, USA)
FOX-P3b Mouse IgG1 236A/E7/PE 50 eBioscience (San Diego, CA, USA)
CCR4b Mouse IgG1,κ 1G1/PE-Cy7 200 BD Pharmingen™ (Basel, Switzerland)
CCR6 (CD196)b Mouse IgG1,κ 11A9/PE 200 BD Pharmingen™ (Basel, Switzerland)
  1. aAntibodies used to phenotype bone marrow-derived mensenchymal stem cells (BMSCs) by flow cytometry. bAntibodies used to detect CCR4+CCR6+ CD4+ T-helper (Th) cells and forkhead box P3-positive regulatory T (Treg) cells by flow cytometry. PBMC, peripheral blood mononuclear cell.