Effect of anti-TIM-2 mAbs on Ig production in vitro. Purified B cells from FcRγ-deficient mice were stimulated with anti-IgM, anti-CD40, and IL-4 in the presence of the indicated anti-T cell immunoglobulin and mucin domain (TIM)-2 monoclonal antibodies (mAbs) or control IgG. Culture supernatants were harvested at day 7 and analyzed using a CBA assay that could identify all seven mouse Ig isotypes in a single sample. Presence of a particular isotype is indicated by lambda (upper) and kappa (lower) fluorescence (with labels provided to indicate positive cultures). Data are representative of three experiments.