Early response genes induced in chondrocytes stimulated with the inflammatory cytokine interleukin-1beta

Table 1 PCR primers and conditions

			Annealing		Product
Gene	Primers		temperature	Cycles	length^*
MMP-13	CCAGTGGTGGTGATGAAGATG	Sense	52°C	25	143
	TTCTCGGAGCCTCTCAGTC	Antisense
c-Jun	GGAAACGACCTTCTATGACGATGCCCTCAA	Sense	63°C	22	316
	GAACCCCTCCTGCTCATCTGTCACGTTCTT	Antisense
JunD	GAGTGTTCGATTCTGCCCTA	Sense	53°C	22	214
	TGTGGACTCGTAGCAAAACA	Antisense
JunB	CCAGTCCTTCCACCTCGACGTTTACAAG	Sense	58°C	22	257
	GACTAAGTGCGTGTTTCTTTTCCACAGTAC	Antisense
egr-1	TCCCGGCTCCTCGACCTAC	Sense	60°C	25	177
	AAGGTTGCTGTCATGTCCGAAAG	Antisense

GAPDH was amplified using Clontech's human GPDH amplimer set with an annealing temperature of 58°C and 25 cycles of amplification. MMP-13 = matrix metalloproteinase-13; egr-1 = early growth response gene-1. ^*Measured in base pairs.

ISSN: 1478-6362