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Figure 7 | Arthritis Research & Therapy

Figure 7

From: A folate receptor beta-specific human monoclonal antibody recognizes activated macrophage of rheumatoid patients and mediates antibody-dependent cell-mediated cytotoxicity

Figure 7

m909 induces ADCC in FRβ-positive cells but not in FRβ-negative cells. Freshly isolated PBMCs were incubated with target cells (CHO-hFRβ, PreB L1.2, or CHO-K1) at a ratio of 50:1 in the presence of m909 IgG1 at three different concentrations (8, 40, or 200 nM) or an isotype control IgG1 at 200 nM (first set of columns). ADCC was detected with CytoTox-ONE reagent, allowing measurement of the lactate dehydrogenase released by lysed target cells. The percentage of specific lysis was calculated as described in Materials and methods. Dark bar indicates preB L1.2 cells, gray bar indicates CHO-hFRβ cells, and clear bar indicates CHO-K1 cells. ADCC, antibody-dependent cell-mediated cytotoxicity; FRβ, folate receptor beta; PBMC, peripheral blood mononuclear cell.

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Arthritis Research & Therapy

ISSN: 1478-6362

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