Figure 5

The effects of trichostatin A on bone marrow-derived dendritic cells. (A) The effects of trichostatin A on cytokine production by bone marrow-derived dendritic cells. Bone marrow-derived dendritic cells were generated from SKG mice and stimulated for 18 h with zymosan A and/or triclostatin A. The concentrations of IL-12p70, IL-12p40, and IL-6 in the supernatant were measured by an enzyme-linked immunosorbent assay. Values are presented as the mean ± SE (n = 3). Data are representative of two (IL-12p70) or three (IL-12p40 and IL-6) independent experiments with similar results (P = 0.027 in IL-12p70, P = 0.024 in IL-12p40, P = 0.029 in IL-6). (B) The effects of triclostatin A on indoleamine 2,3-dioxygenase mRNA expression by bone marrow-derived dendritic cells. Bone marrow-derived dendritic cells were generated from SKG mice and stimulated for 18 h with zymosan A (5 μg/ml) and/or trichostatin A (20 nM). IDO1 and IDO2 mRNA expression was measured by quantitative real-time polymerase chain reaction. Representative results of two independent experiments are shown. (C) The effect of trichostatin A on indoleamine 2,3-dioxygenase production by bone marrow-derived dendritic cells. Bone marrow-derived dendritic cells were stimulated with zymosan A (5 μg/ml) and/or trichostatin A (20 nM) for 48 h. Cell lysates were analyzed by Western blotting with anti- indoleamine 2,3-dioxygenase antibodies. The blot is representative of two independent experiments.