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Table 2 Fold changes in gene expression data for media conditions B, C, NCA and NCT Significance greater than two-fold relative to Day 0 MSCs or Basal in bold and underlined; + = fold increase & - = fold decrease (P < 0

From: Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells

ALL GENES Normalized to Day 0 Normalized to Basal
    B C NCA NCT C NCA NCT
   BGN -1.0 +1.1 -6.1 +1.6 +1.1 -6.2 +1.6
   GPC1 +2.5 +2.1 +1.5 +1.9 -1.2 -1.7 -1.2
  IVD KRT19 -7.3 -1.0 -1.4 -12.7 -1.0 +1.1 -1.6
Phenotypic   LAMB1 +1.6 +1.3 -1.2 +1.3 -1.3 -2.0 -1.2
   SOX9 +2.59 +1.48 -1.32 +6.79 -1.65 -3.49 +2.74
  MSC BGLAP +2.02 -1.13 +1.95 +3.51 -2.37 -1.12 +1.58
   PPARG +27.62 +9.90 +9.53 +19.86 -2.79 +1.24 -1.41
   ACAN -14.1 -6.3 -2.5 -16.2 +2.1 -1.2 -1.2
   COL1A1 -1.1 +1.2 -29.7 -3.4 +1.3 -27.3 -2.9
   COL10A1 +1.1 +60.4 +2.2 +61.9 +52.1 +1.7 +51.8
Matrix proteins COL2A1 -1.0 +49.2 -1.0 +15.4 +32.2 -1.0 +8.1
   COL3A1 +3.6 +3.9 -11.7 -2.2 +1.1 -42.9 -7.4
   eln -1.4 +12.9 -3.0 -2.4 +17.4 -20.8 -1.8
   HAS1 -7.3 +1.3 +1.0 +1.1 11.4 +7.5 +9.3
  Aggrecanases ADAMTS4 +6.4 +4.9 +2.2 +26.4 -1.3 -2.9 +4.2
   ADAMTS5 +1.1 -2.5 +3.2 +5.1 -2.6 +2.9 +5.2
Matrix enzyme   MMP1 +369.3 +9.7 +86039.1 +28040.4 -3.2 +231.6 +78.2
  MMPs MMP13 +2637.8 +3056.0 +1085.0 +15582.2 +1.1 -2.5 +6.0
   MMP14 +4.5 +2.2 +6.2 +6.8 -2.1 +1.3 +1.5
   MMP2 +3.8 +1.8 +4.7 +4.0 -2.1 +1.2 +1.0
   MMP3 +36.1 +184.4 +203663.7 +1973.3 +11.7 +12436.5 +120.9
   MMP9 +22.2 +5.9 +19.1 +3.2 -3.8 -1.1 -7.3
   TIMP1 +4.99 +1.77 +5.90 +9.12 -2.60 +1.26 +1.79
Anti-catabolic TIMP2 +1.46 -1.10 +1.26 +1.66 -1.64 -1.09 +1.14
   TIMP3 -1.00 -1.00 -1.00 +3.68 -1.00 -1.00 +3.08
   TGFB1 +1.82 +1.92 +2.01 +3.96 +1.06 +1.07 +2.11
   TGFB2 +1.43 -3.34 -4.91 -1.80 -4.86 -6.78 -2.49
Growth factors TGFB TGFB3 +8.31 +1.36 +3.08 +5.27 -6.23 -2.97 -1.58
   TGFBR1 -4.52 -4.61 -1.03 +1.05 -1.00 +4.19 +4.83
   TGFBR2 +1.03 +1.65 +1.25 +1.17 1.59 +1.18 +1.11
   CTGF -8.9 -3.9 -76.5 -9.6 +2.4 -8.2 -1.0
   EGF -3.3 -7.1 -1.1 -1.3 -2.1 +1.5 +2.4
   FGF1 -1.6 -1.5 +1.6 +9.2 +1.1 +2.4 +14.1
  General IGF1 +169.7 +104.0 -1.0 +87.4 -1.6 -1.0 -1.9
   PDGFA -1.63 -1.57 -1.39 -1.06 +1.02 +1.15 +1.46
   WISP1 +3.27 +2.62 -2.44 +1.90 -1.35 -8.23 -1.75
   IL1B +22.7 +15.1 +9911.6 +444.5 -2.1 +310.6 +14.7
   TNF +1.01 +1.66 +90.93 +5.33 +1.56 +72.86 +3.32
Inflammatory CASP3 +1.3 -1.0 -1.2 +1.2 -1.3 -1.8 -1.1
   BDNF -12.9 -4.7 -1.3 -6.3 -1.2 +3.1 +2.0
   NGF -4.0 -2.7 +1.4 -4.4 -1.6 +1.6 -1.1
   TAC4 -1.08 +1.03 +4.22 +1.28 +1.09 +4.33 +1.36