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Table 2 IL-17 in CD4+ T cells: Comparison of ELISA and intracellular cytokine staininga

From: Analysis of IL-17+cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response

Individuals ELISA for IL-17-secreting CD4+ T cells Intracellular cytokine staining for CD4+IL-17+ T cells
  Without stimulation With PMA/ionomycin Without stimulation With PMA/ionomycin
AS patient 1 0 pg/nL 1,667.9 pg/nL 0.0% of CD4+ T cells 0.98% of CD4+ T cells
AS patient 2 0 pg/nL 967.8 pg/nL 0.0% of CD4+ T cells 0.47% of CD4+ T cells
AS patient 3 0 pg/nL 1,398.5 pg/nL 0.0% of CD4+ T cells 1.72% of CD4+ T cells
Control 1 0 pg/nL 1,784.4 pg/nL 0.0% of CD4+ T cells 1.21% of CD4+ T cells
Control 2 0 pg/nL 277.9 pg/nL 0.0% of CD4+ T cells 0.57% of CD4+ T cells
Control 3 0 pg/nL 920.5 pg/nL 0.0% of CD4+ T cells 0.69% of CD4+ T cells
  1. aPMA = phorbol 12-myristate 13-acetate; AS = ankylosing spondylitis.