Skip to main content

Table 2 IL-17 in CD4+ T cells: Comparison of ELISA and intracellular cytokine staininga

From: Analysis of IL-17+cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response

Individuals

ELISA for IL-17-secreting CD4+ T cells

Intracellular cytokine staining for CD4+IL-17+ T cells

 

Without stimulation

With PMA/ionomycin

Without stimulation

With PMA/ionomycin

AS patient 1

0 pg/nL

1,667.9 pg/nL

0.0% of CD4+ T cells

0.98% of CD4+ T cells

AS patient 2

0 pg/nL

967.8 pg/nL

0.0% of CD4+ T cells

0.47% of CD4+ T cells

AS patient 3

0 pg/nL

1,398.5 pg/nL

0.0% of CD4+ T cells

1.72% of CD4+ T cells

Control 1

0 pg/nL

1,784.4 pg/nL

0.0% of CD4+ T cells

1.21% of CD4+ T cells

Control 2

0 pg/nL

277.9 pg/nL

0.0% of CD4+ T cells

0.57% of CD4+ T cells

Control 3

0 pg/nL

920.5 pg/nL

0.0% of CD4+ T cells

0.69% of CD4+ T cells

  1. aPMA = phorbol 12-myristate 13-acetate; AS = ankylosing spondylitis.