Analysis of IL-17 + cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response

Table 2 IL-17 in CD4⁺ T cells: Comparison of ELISA and intracellular cytokine staining^a

Individuals	ELISA for IL-17-secreting CD4⁺ T cells		Intracellular cytokine staining for CD4⁺IL-17⁺ T cells
	Without stimulation	With PMA/ionomycin	Without stimulation	With PMA/ionomycin
AS patient 1	0 pg/nL	1,667.9 pg/nL	0.0% of CD4⁺ T cells	0.98% of CD4⁺ T cells
AS patient 2	0 pg/nL	967.8 pg/nL	0.0% of CD4⁺ T cells	0.47% of CD4⁺ T cells
AS patient 3	0 pg/nL	1,398.5 pg/nL	0.0% of CD4⁺ T cells	1.72% of CD4⁺ T cells
Control 1	0 pg/nL	1,784.4 pg/nL	0.0% of CD4⁺ T cells	1.21% of CD4⁺ T cells
Control 2	0 pg/nL	277.9 pg/nL	0.0% of CD4⁺ T cells	0.57% of CD4⁺ T cells
Control 3	0 pg/nL	920.5 pg/nL	0.0% of CD4⁺ T cells	0.69% of CD4⁺ T cells

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