Individuals
|
ELISA for IL-17-secreting CD4+ T cells
|
Intracellular cytokine staining for CD4+IL-17+ T cells
|
---|
|
Without stimulation
|
With PMA/ionomycin
|
Without stimulation
|
With PMA/ionomycin
|
---|
AS patient 1
|
0 pg/nL
|
1,667.9 pg/nL
|
0.0% of CD4+ T cells
|
0.98% of CD4+ T cells
|
AS patient 2
|
0 pg/nL
|
967.8 pg/nL
|
0.0% of CD4+ T cells
|
0.47% of CD4+ T cells
|
AS patient 3
|
0 pg/nL
|
1,398.5 pg/nL
|
0.0% of CD4+ T cells
|
1.72% of CD4+ T cells
|
Control 1
|
0 pg/nL
|
1,784.4 pg/nL
|
0.0% of CD4+ T cells
|
1.21% of CD4+ T cells
|
Control 2
|
0 pg/nL
|
277.9 pg/nL
|
0.0% of CD4+ T cells
|
0.57% of CD4+ T cells
|
Control 3
|
0 pg/nL
|
920.5 pg/nL
|
0.0% of CD4+ T cells
|
0.69% of CD4+ T cells
|
- aPMA = phorbol 12-myristate 13-acetate; AS = ankylosing spondylitis.