Figure 6

Regulation of TLR3 expression in RA FLSs stimulation by various inflammatory factors. (A) FLSs from RA patients (n = 3) were stimulated with TNF-α, IL-1β, IFN-α, IFN-β, PGN, poly(I:C) and LPS for 24 hours, and TLR3 expression was regulated to different extents. Values are mean ± SEM fold induction of TLR3 mRNA relative to unstimulated cells (set as control). Baseline expression (control group) was set at 1. (B) Induction of TLR3 mRNA expression in FLSs from RA patients (n = 3) and OA patients (n = 3) with series poly(I:C) dose stimulation. Data are presented as means ± SEM from three independent experiments. Levels of significance were calculated by using Student's t-test (*P < 0.05, ***P < 0.001).