Table 1 Primer information for real-time PCR

tlr1 (rat)

[NM_001172120]

Forward

CAGCAGCCTCAAGCATGTCTA

82

60

Reverse

CAGCCCTAAGACAACAATACAATAGAAGA

tlr2 (rat)

[NM_198769]

Forward

CTCCTGTGAACTCCTGTCCTT

74

60

Reverse

AGCTGTCTGGCCAGTCAAC

tlr3 (rat)

[NM_198791]

Forward

GATTGGCAAGTTATTCGTC

205

54

Reverse

GCGGAGGCTGTTGTAGG

tlr4 (rat)

[NM_019178]

Forward

GATTGCTCAGACATGGCAGTTTC

135

54

Reverse

CACTCGAGGTAGGTGTTTCTGCTAA

tlr5 (rat)

[NM_001145828]

Forward

GGGCAGCAGAAAGACGGTAT

61

60

Reverse

CAGGCACCAGCCATCCTTAA

tlr6 (rat)

[NM_207604]

Forward

AGAACCTTACTCATGTCCCAAAAGAC

79

60

Reverse

AGATCAGATATGGAGTTTTGAGACAGACT

tlr7 (rat)

[NM_001097582]

Forward

GTTTTACGTCTACACAGTAACTCTCTTCA

75

60

Reverse

TTCCTGGAGGTTGCTCATGTTTT

tlr8 (rat)

[NM_001101009]

Forward

GGGGTAACACACCGTCTA

150

60

Reverse

GTCAAGGCGATTTCCACT

tlr9 (rat)

[NM_198131]

Forward

CCGAAGACCTAGCCAACCT

70

60

Reverse

TGATCACAGCGACGGCAATT

ifn-β (rat)

[NM_019127]

Forward

CTTGGGTGACATCCACGACTAC

92

54

Reverse

GGCATAGCTGTTGTACTTCTTGTCTT

il-6 (rat)

[NM_012589]

Forward

AAGAAAGACAAAGCCAGAGTC

263

60

Reverse

CACAAACTGATATGCTTAGGC

mmp3(rat)

[NM_133523]

Forward

ATCCCCTGATGTCCTCG

147

54

Reverse

TTTCGCCAAAAGTGCC

mmp13 (rat)

[NM_133530]

Forward

TTCAACCCTGTTTACCT

293

54

Reverse

TTCTTTTTCCTTGTCCC

β-actin (rat)

[NM_031144]

Forward

GAGGGAAATCGTGCGTGAC

157

60

Reverse

GCATCGGAACCGCTCATT

TLR3 (human)

[NM_003265]

Forward

AGCCTTCAACGACTGATGCT

201

60

Reverse

TTTCCAGAGCCGTGCTAAGT

β- ACTIN (human)

[NM_001101]

Forward

AGTTGCGTTACACCCTTTCTTG

150

60

Reverse

TCACCTTCACCGTTCCAGTTT