Figure 2
The mRNA of RAGE was increased by IL-17 in a dose-dependent manner in rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS). (a) RA-FLS were cultured with the indicated doses of IL-17 for 18 h. Total mRNA was extracted and analyzed by real-time PCR with SYBR Green I. Values are the mean ± SEM from one representative experiment with FLS from four patients with RA. RA-FLS (2 × 105) were cultured with IL-17 for 18 h. Cell viability was assessed by lactate dehydrogenase (LDH) activity. (b) FLS were treated with the same method as (a). RAGE expression in the FLS was determined using a RAGE-specific antibody. (c) RA-FLS were cultured with the indicated doses of IL-17 for 48 h. RAGE was assessed by ELISA. Values are the mean ± SEM from one representative experiment with FLS from four patients with RA. *P < 0.05, **P < 0.01 compared to untreated cells.