Table 1 Multiple reaction monitoring transitions for liquid chromatography-tandem mass spectrometry assay of eicosanoids
From: A targeted lipidomics approach to the study of eicosanoid release in synovial joints
Compound | MRM, m/z | Collision energy, eV | Declustering potential |
|---|---|---|---|
PGE1 | 353 → 317 | -30 | -80 |
6-keto PGF1α | 369 → 163 | -35 | -100 |
PGD2 | 351 → 271 | -25 | -40 and -80 |
PGE2 | 351 → 271 | -25 | -40 and -80 |
|  | 351 → 175 | -30 | -80 |
PGF2α | 353 → 193 | -35 | -90 |
11β-PGF2α | 353 → 309 | -20 | -60 |
PGF2β | 353 → 309 | -25 | -40 |
PGJ2 | 333 → 189 | -25 | -40 |
|  | 333 → 271 | -25 | -80 |
15-deoxy-Δ-12,14 PGJ2 | 315 → 271 | -20 | -90 |
13,14-dihydro-15-keto PGD2 | 351 → 207 | -27 | -80 |
13,14-dihydro-15-keto PGE2 | 351 → 333 | -17 | -80 |
13,14-dihydro-15-keto PGF2α | 353 → 113 | -40 | -100 |
TXB2 | 369 → 195 | -23 | -80 |
|  | 369 → 169 | -25 | -90 |
LTB4 | 335 → 195 | -20 | -100 |
5(S)-HETE | 319 → 115 | -20 | -40 |
8(S)-HETE | 319 → 155 | -20 | -80 |
11(S)-HETE | 319 → 167 | -20 | -80 |
12(S)-HETE | 319 → 179 | -20 | -80 |
15(S)-HETE | 319 → 175 | -20 | -80 |
LTD4 | 495 → 177 | -30 | -100 |
LTE4 | 438 → 333 | -25 | -100 |
LXA4 | 351 → 235 | -20 | -80 |
16,16-dimethyl PGF2α (IS) | 381 → 319 | -35 | -100 |