Immunoprecipitated Western blots and autoantibody profiles for 11 SSc patients. Each SSc patient (SSc1 to 11) has an age and sex matched normal control (C1 to 11) for comparison of sumoylated topo I expression with IP Western blots. Poly-sumoylated topo I appeared in SSc fibroblast strain number 1, 2, 3, 4, 7 and 9. Increased sumoylation of topo I also is observed in the case number 6 compared to its normal counterpart, but not in the case number 5, 8, 10 and 11. ANA, antinuclear antibodies.