| | | | | |
100 pM
|
100 nM
|
|---|
| |
UT
|
IL-1β
|
CNP
|
IL-1β + CNP
|
IL-1β + CNP + KT5823
|
CNP
|
IL-1β + CNP
|
IL-1β + CNP + KT5823
|
|---|
|
Culture period (hr)
|
6
|
48
|
6
|
48
|
6
|
48
|
6
|
48
|
6
|
48
|
6
|
48
|
6
|
48
|
6
|
48
|
|
Collagen type I
|
a
|
a
|
ND
|
ND
|
↑b
|
↑c
|
ND
|
ND
|
ND
|
ND
|
a
|
↓d
|
ND
|
ND
|
ND
|
ND
|
|
Collagen type II
|
↑b
|
a
|
↓d
|
↓d
|
a
|
a
|
a
|
a
|
a
|
a
|
a
|
↑c
|
↓d
|
↓d
|
a
|
a
|
|
Col X
|
a
|
a
|
a
|
a
|
↑b
|
↑c
|
↑ b
|
↑ c
|
↓ d
|
↓d
|
a
|
a
|
a
|
a
|
a
|
a
|
|
Sox-9
|
a
|
a
|
ND
|
ND
|
a
|
a
|
ND
|
ND
|
ND
|
ND
|
↑b
|
↑c
|
ND
|
ND
|
ND
|
ND
|
|
MMP-13
|
a
|
a
|
↑b
|
↑c
|
↑b
|
↑b
|
↑b
|
↑c
|
↓d
|
↓d
|
a
|
a
|
↓d
|
↓d
|
a
|
a
|
- aNo significant change; ↑b, a significant increase (P < 0.05); ↑c, a significant increase (P < 0.01); ↓d, a significant decrease (P < 0.05); ND, not determined. Chondrocyte/agarose constructs were cultured with low (100 pM) or high (100 nM) concentrations of CNP with 0 or 10 ng/ml IL-1β and/or 5 μM KT5823 for 6 and 48 hours (n = 8). Note that statistical comparisons were determined for the following samples: Time = 0 versus 6 or 48 hr (UT); UT versus IL-1β; UT versus CNP; IL-1β versus IL-1β + CNP; IL-1β + CNP versus IL-1β + CNP + KT5823.
