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Table 2 Effect of CNP on gene expression in chondrocyte/agarose constructs

From: Biomechanical signals and the C-type natriuretic peptide counteract catabolic activities induced by IL-1β in chondrocyte/agarose constructs

      100 pM 100 nM
  UT IL-1β CNP IL-1β + CNP IL-1β + CNP + KT5823 CNP IL-1β + CNP IL-1β + CNP + KT5823
Culture period (hr) 6 48 6 48 6 48 6 48 6 48 6 48 6 48 6 48
Collagen type I a a ND ND b c ND ND ND ND a d ND ND ND ND
Collagen type II b a d d a a a a a a a c d d a a
Col X a a a a b c b c d d a a a a a a
Sox-9 a a ND ND a a ND ND ND ND b c ND ND ND ND
MMP-13 a a b c b b b c d d a a d d a a
  1. aNo significant change; ↑b, a significant increase (P < 0.05); ↑c, a significant increase (P < 0.01); ↓d, a significant decrease (P < 0.05); ND, not determined. Chondrocyte/agarose constructs were cultured with low (100 pM) or high (100 nM) concentrations of CNP with 0 or 10 ng/ml IL-1β and/or 5 μM KT5823 for 6 and 48 hours (n = 8). Note that statistical comparisons were determined for the following samples: Time = 0 versus 6 or 48 hr (UT); UT versus IL-1β; UT versus CNP; IL-1β versus IL-1β + CNP; IL-1β + CNP versus IL-1β + CNP + KT5823.