Figure 5
From: Fragment of tegument protein pp65 of human cytomegalovirus induces autoantibodies in BALB/c mice
Detection of anti-nuclear reactivity in pp65 1-167 or pp65 336-439 specific antibodies from eight weeks post-immunization sera. (a) ELISA assays of affinity purified anti-pp65336-439 antibody (pp65336-439), anti-pp651-167 antibody (pp651-167), SLE patients' sera (SLE) and flow through against purified HCMV virions. Graphs depict mean ± s.e.m. values. Unpaired Student t test was performed. Results with a P-value of < 0.05 were considered to be significant. (b) Immunoblot assays on affinity purified anti-pp65336-439 antibody (pp65336-439), anti-pp651-167 antibody (pp651-167), SLE patients' sera (SLE) and flow through against purified HCMV virions. Molecular mass markers (kD) are shown on the left. MW: molecular weight. (c) ANA stains with HEp-2 substrate slides were performed with HCMV affinity-purified antibody. Patterns of speckle (c 1 , c 7 ), chromatin (c 2 , c 8 ), centriole (c 3 , c 9 ) and MSA II (c 4 , c 10 ) were revealed with affinity purified anti-pp65336-439 antibodies. Nuclear pattern was not found in flow through (c 5 , c 11 ) or affinity-purified anti-pp651-167 antibody (c 6 , c 12 ) stains. White arrowheads indicate the patterns of nuclear response. MSA II: mitotic spindle type II.