Figure 4

Graphical representation of Ingenuity Pathways Analysis. An IPA of research literature-based molecular relationships among protein components identified by multivariate Random Forest (RF) modeling (red) which describes possible interactions accounting for the accurate classification of affected vs. unaffected MZ twins discordant for SAID. The IPA Grow, Connect, and Path Explorer software functions were used to establish a spatial model utilizing the Shortest Pathway option. A minimum of two nodes (that is, interconnecting molecules), shown in yellow, were required to integrate the seven protein markers identified by RF analysis.