Figure 1From: Lymphotoxin-beta receptor blockade reduces CXCL13 in lacrimal glands and improves corneal integrity in the NOD model of Sjögren's syndrome Number of leukocytes in lacrimal glands is reduced by treatment with LTBR-Ig. Representative photomicrographs of hematoxylin and eosin stained paraffin tissue-sections (a to c) and tissue-sections stained with anti-B220 and immuno-peroxidase (dark brown) (d to h) from NOD mice that were not treated (NT) or were treated from 8 to 16 weeks of age with MOPC-21 control antibody or with LTBR-Ig. Arrows (a,b) denote aggregations of leukocyte nuclei (dark blue). Bar equals 100 micron. Specificity control for anti-B220 staining of a section from a untreated mouse lacrimal gland (g); the area of the inset box in (g) is shown at higher magnification in (h) or an adjacent section stained without the primary antibody (i); arrowhead marks same location in (h,i). FACS analysis of leukocytes isolated from lacrimal glands of mice treated with MOPC-21 control antibody (n = 8 glands) or LTBR-Ig (n = 8 glands) (j). Mean and standard deviation of total CD45 positive leukocytes, total number of B220 positive cells (B-cells) and of CD3 positive cells (T-cells) are indicated for each treatment type. This experiment was repeated four times. Statistical significance was calculated by two-tailed T-test. FACS, flow cytometry; LTBR-Ig, lymphotoxin-beta receptor-mouse immunoglobulin (Fc), NOD, non-obese diabetic.Back to article page