Table 2 Characteristics of satellite cell strains obtained from rheumatoid arthritis patients and osteoarthritis patients
From: Regenerative potential of human muscle stem cells in chronic inflammation
Characteristic | RA N= 4 | OA N= 8 | β | 95% CI | P-value |
|---|---|---|---|---|---|
Myogenicity (% desmin positive cells) | |||||
After explantation | 46 (13) | 58 (26) | -0.35 | -51 to 19 | 0.32 |
After CD56 selection | 87 (5) | 95 (3) | -0.72 | -13 to -2 | 0.02 |
After thawing at early replicative phase* | 60 (20) | 47 (32) | -0.57 | -209 to 142 | 0.62 |
At mid-term replicative phase# | 56 (37) | 37 (33) | -0.02 | -49 to 46 | 0.95 |
At maximum replicative capacity | 47 (43) | 38 (30) | -0.07 | -86 to 77 | 0.90 |
After oxidative stress† | 21 (12) | 16 (10) | 0.21 | -341 to 349 | 0.91 |
Viability (% alive cells) | |||||
At early replicative phase* | 91 (4) | 94 (2) | -0.39 | -8 to 4 | 0.33 |
After oxidative stress† | 33 (24) | 34 (24) | 0.48 | -114 to 153 | 0.31 |
Growth characteristics* | |||||
Proliferation index‡ (% BrdU positive cells) | 86 (2) | 85 (6) | -0.05 | -10 to 9 | 0.89 |
Colony formation (number) | 78 (44) | 85 (34) | -0.12 | -83 to 66 | 0.78 |
Myogenic colonies (%) | 30 (16) | 27 (31) | -0.40 | -41 to 7 | 0.14 |
Growth speed (PD/day) | 0.39 (0.10) | 0.32 (0.08) | 0.310.28 | -0.07 to 0.18-5.0 to 13.9 | 0.290.28 |
Maximum replicative capacity (PD) | 33.4 (9.7) | 27.4 (5.5) | -0.29 | -385 to 359 | 0.73 |
Proliferation index after oxidative stress†‡ (% BrdU positive cells) | 15 (15) | 34 (24) | |||
Fusion index at early replicative phase*§ (% of fused myonuclei) | 27 (12) | 25 (13) | -0.01 | -26 to 26 | 0.99 |
Normalized protein expression* | |||||
p16 | 103 (15) | 140 (63) | -0.62 | -85 to 7 | 0.08 |
p21 | 27 (4) | 27 (5) | -0.28 | -13 to 8 | 0.60 |
p53 | 67 (12) | 62 (8) | -0.18 | -18 to 11 | 0.58 |
hTERT | 32 (6) | 39 (14) | -0.26 | -23 to 11 | 0.39 |
Relative telomere length* (% of ref. cell line 1301) | 22 (5) | 23 (10) | -0.46 | -16 to -0.4 | 0.04 |