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Figure 1 | Arthritis Research & Therapy

Figure 1

From: Extracellular nicotinamide phosphoribosyltransferase (NAMPT/visfatin) inhibits insulin-like growth factor-1 signaling and proteoglycan synthesis in human articular chondrocytes

Figure 1

Extracellular nicotinamide phosphoribosyltransferase inhibits insulin-like growth factor-1-stimulated proteoglycan synthesis. (A) Normal human chondrocytes were treated with (0 to 5 μg/ml) extracellular nicotinamide phosphoribosyltransferase (eNAMPT)/visfatin overnight, followed by 24-hour stimulation with 50 ng/ml insulin-like growth factor-1 (IGF-1). (B) Cells were stimulated with 5 μg/ml eNAMPT or 50 ng/ml IGF-1 overnight. Proteoglycan (PG) synthesis was measured using [35S]sulfate incorporation and DNA was analyzed with a PicoGreen assay. Results are expressed as the fold change versus unstimulated control. Data presented as mean ± standard deviation of three independent experiments.

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