Overall profiling of cytokines in joints and bloodstream of rats developing antigen-induced arthritis. Synovial fluid of both knees - sensitized with methylated bovine serum albumin (mBSA) and saline-injected - and sera were collected at necropsy, and mediator levels were measured by multiplex immunoassay. Results are expressed as fold of induction or of inhibition in comparison with day of sensitization (day 0) (n = 5, representative of three independent experiments). Blue squares indicate that the corresponding mediator was at the basal level, dark red squares indicate the maximal induction fold (50×), and purple squares indicate the maximal inhibition level (/5). D, day; G-CSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte macrophage colony-stimulating factor; GRO/KC, growth-related oncogene/keratinocyte chemoattractant; H, hour; IFNγ, interferon gamma; IL, interleukin; IP-10, inducible protein-10; MCP-1, monocyte chemoattractant protein-1; MIP-1α, macrophage inflammatory protein-1-alpha; RANTES, regulated on activation normal T expressed and secreted; TNF-α, tumor necrosis factor-alpha; VEGF, vascular endothelial cell growth factor.