Cytokines profiling by multiplex analysis in experimental arthritis: which pathophysiological relevance for articular versus systemic mediators?

Table 1 Changes in cytokine mRNA level (fold change relative to day 0) in arthritic synovium during antigen-induced arthritis

Time point	MCP-1		IL-1β		TNF-α		VEGF		IL-6
	Sensitized	Saline-injected	Sensitized	Saline-injected	Sensitized	Saline-injected	Sensitized	Saline-injected	Sensitized	Saline-injected
Day 0	1.0 ± 0.2	1.0 ± 0.1	1.0 ± 0.1	1.0 ± 0.2	1.0 ± 0.4	1.0 ± 0.5	1.0 ± 0.1	1.0 ± 0.2	1.0 ± 0.2	1.0 ± 0.1
7 hours	13.9^a ± 5.4	1.5 ± 0.6	30.2^a ± 4.7	1.1 ± 0.2	5.13^a ± 2.4	1.2 ± 0.5	3.8^a ± 0.5	1.1 ± 0.2	35.0^a ± 8.5	2.1 ± 1.0
Day 1	5.9^a ± 1.9	1.1 ± 0.2	49.6^a ± 29.4	1.0 ± 0.2	11.7^a ± 5.2	1.1 ± 0.6	20.0^a ± 12.2	1.1 ± 0.3	12.2^a ± 2.6	1.2 ± 0.3
Day 2	6.3^a ± 2.5	1.2 ± 0.4	23.4^a ± 2.0	1.5 ± 0.5	0.4 ± 0.3	1.3 ± 0.7	3.7^a ± 0.6	1.1 ± 0.3	157.5^a ± 21.2	1.2 ± 0.3
Day 3	7.2^a ± 2.7	1.1 ± 0.2	11.0* ± 2.7	1.1 ± 0.2	0.7 ± 0.5	1.1 ± 0.6	2.0 ± 0.4	1.0 ± 0.2	58.2^a ± 6.4	1.4 ± 0.6
Day 7	3.3^a ± 0.7	1.0 ± 0.2	5.9 ± 2.3	1.1 ± 0.2	0.3 ± 0.2	1.2 ± 0.6	0.7 ± 0.3	1.0 ± 0.2	79.7^a ± 42.2	1.1 ± 0.3
Day 14	3.2^a ± 1.4	1.1 ± 0.2	2.3 ± 0.3	1.0 ± 0.1	0.8 ± 0.5	1.1 ± 0.5	0.7 ± 0.2	1.0 ± 0.1	28.7^a ± 7.1	1.3 ± 0.5

Rats were immunized subcutaneously with 0.5 g of methylated bovine serum albumin (mBSA) emulsified with complete Freund's adjuvant 21 and 14 days prior to articular injection (day 0) of 0.5 mg of mBSA (sensitized) or 50 μL of saline (contralateral) into the knee joints. Expression of mRNA was assessed before (on day 0) and after (at 7 hours and on days 1, 2, 3, 7, and 14) sensitization. Data are expressed as delta delta threshold cycle (Ct) related to the housekeeping gene (RP29). Values are the mean ± standard error of the mean of five independent measurements per time. ^aP < 0.05 in comparison with day 0. IL, interleukin; MCP-1, monocyte chemoattractant protein-1; TNF-α, tumor necrosis factor-alpha; VEGF, vascular endothelial cell growth factor.

ISSN: 1478-6362