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Table 1 Primer sequences used for real-time PCR

From: miR-146a, an IL-1β responsive miRNA, induces vascular endothelial growth factor and chondrocyte apoptosis by targeting Smad4

 

Sense primer

Antisense primer

Rat β-actin [GenBank:NM_031144.2]

5'-CTCTTCCAGCCTTCCTTCCT-3'

5'-TCATCGTACTCCTGCTTGCT-3'

Rat U6 [GenBank:K00784]

5'-CTCGCTTCGGCAGCACA-3'

5'-AACGCTTCACGAATTTGCGT-3'

Rat Smad4 [GenBank:NM_019275.2]

5'-CCACCAACTTCCCCAACATT-3'

5'-TGCAGTCCTACTTCCAGTCCAG-3'

Rat VEGF [GenBank:NM_031836.2]

5'-TTGAGACCCTGGTGGACATCT-3'

5'-CTCCTATGTGCTGGCTTTGG-3'

  1. VEGF, vascular endothelial growth factor.
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Arthritis Research & Therapy

ISSN: 1478-6362

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