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Table 1 Primer sequences used for real-time PCR

From: miR-146a, an IL-1β responsive miRNA, induces vascular endothelial growth factor and chondrocyte apoptosis by targeting Smad4

  Sense primer Antisense primer
Rat β-actin [GenBank:NM_031144.2] 5'-CTCTTCCAGCCTTCCTTCCT-3' 5'-TCATCGTACTCCTGCTTGCT-3'
Rat U6 [GenBank:K00784] 5'-CTCGCTTCGGCAGCACA-3' 5'-AACGCTTCACGAATTTGCGT-3'
Rat Smad4 [GenBank:NM_019275.2] 5'-CCACCAACTTCCCCAACATT-3' 5'-TGCAGTCCTACTTCCAGTCCAG-3'
Rat VEGF [GenBank:NM_031836.2] 5'-TTGAGACCCTGGTGGACATCT-3' 5'-CTCCTATGTGCTGGCTTTGG-3'
  1. VEGF, vascular endothelial growth factor.