Binding of ACT1 and its mutant variants to TNF receptor-associated factor (TRAF)6 and interleukin (IL)-17RA. Interaction of wild-type TRAF3IP2/ACT1 and its mutant variants with either TRAF6, upper panel, or IL-17RA, lower panel, was determined by mammalian two-hybrid system. The TRAF3IP2 mutant p.Asp10Asn showed a significantly reduced binding to TRAF6. Common coding variants indicated by underlined and italicized letters. *P < 0.005, when compared with wild-type TRAF3IP2.