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Table 1 Primers used in real-time polymerase chain reaction

From: Enhanced susceptibility to lipopolysaccharide-induced arthritis and endotoxin shock in interleukin-32 alpha transgenic mice through induction of tumor necrosis factor alpha

Primer

Sense

Sequence

Mouse GAPDH

Forward

5'-CTTTGTCAAGCTCATTTCCTGG-3'

 

Reverse

5'-TCTTGCTCAGTGTCCTTGC-3'

Human IL-32α

Forward

5'-CCTTGGCTCCTTGAACTTTTG-3'

 

Reverse

5'-CTGTCCACGTCCTGATTCTG-3'

Mouse TNFα

Forward

5'-CTTCTGTCTACTGAACTTCGGG-3'

 

Reverse

5'-CAGGCTTGTCACTCGAATTTTG-3'

Mouse IL-6

Forward

5'-CAAAGCCAGAGTCCTTCAGAG-3'

 

Reverse

5'-GTCCTTAGCCACTCCTTCTG-3'

Mouse MIP-2

Forward

5'-GAAGTCATAGCCACTCTCAAGG-3'

 

Reverse

5'-CTTCCGTTGAGGGACAGC-3'

  1. GAPDH, glyceraldhyde-3-phosphate dehydrogenase; IL, interleukin; MIP-2, macrophage inflammatory protein 2; TNFα, tumor necrosis factor-alpha.
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Arthritis Research & Therapy

ISSN: 1478-6362

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