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Table 1 Sensitivity and specificity of our new IP-qPCR method.

From: A new immunoprecipitation-real time quantitative PCR assay for anti-Th/To and anti-U3RNP antibody detection in systemic sclerosis

Autoantibody specificity a n Anti-Th/To
by IP-qPCR
Anti-U3RNP
by IP-qPCR
Scleroderma
Th/To 22 100% 4.5% (1/22)
U3RNP 12 0% 100%
Topo I 20 0% 0%
RNAPIII 18 0% 0%
ACA 15 0% 0%
PM-Scl 5 0% 0%
Others
TMG 3 0% 100%
La 12 0% 42% (5/12)
NHS 15 0% 0%
  1. Results show the high sensitivity and specificity of the new assay, for the detection of anti-Th/To and -U3RNP antibodies, compared with silver staining as gold standard. aAntibody specificity validated by IP, urea-PAGE, and silver staining. ACA, anti-centromere antibodies; IP, immunoprecipitation; NHS, normal human serum; RNAPIII, RNA polymerase III; TMG, trimethylguanosine; topo I, topoisomerase I.