Autoantibody specificity
a
|
n
|
Anti-Th/To
by IP-qPCR
|
Anti-U3RNP
by IP-qPCR
|
---|
Scleroderma
|
Th/To
|
22
|
100%
|
4.5% (1/22)
|
U3RNP
|
12
|
0%
|
100%
|
Topo I
|
20
|
0%
|
0%
|
RNAPIII
|
18
|
0%
|
0%
|
ACA
|
15
|
0%
|
0%
|
PM-Scl
|
5
|
0%
|
0%
|
Others
|
TMG
|
3
|
0%
|
100%
|
La
|
12
|
0%
|
42% (5/12)
|
NHS
|
15
|
0%
|
0%
|
- Results show the high sensitivity and specificity of the new assay, for the detection of anti-Th/To and -U3RNP antibodies, compared with silver staining as gold standard. aAntibody specificity validated by IP, urea-PAGE, and silver staining. ACA, anti-centromere antibodies; IP, immunoprecipitation; NHS, normal human serum; RNAPIII, RNA polymerase III; TMG, trimethylguanosine; topo I, topoisomerase I.