Figure 3

Real time PCR analysis of expression of osteoblast genes in synovial fluid (SF)-derived cells (A) and peripheral blood mononuclear cells (PBMC) from patients with oligoarticular form of juvenile idiopathic arthritis (oJIA), polyarticular form of JIA (pJIA), and control (ctrl) children. (B) mRNA expression was calculated according to the standard curve for each gene expression in the calibrator sample (cDNA from SF-derived cells or PBMC) and normalized to mRNA quantity for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (endogenous control). Results are presented as median and interquartile range (IQR) for each group; lines represent minimum and maximum values; * P ≤ 0.05 vs. oJIA (Mann-Whitney test). PBMC, peripheral blood mononuclear cell; OPG, osteoprotegerin; RANKL, receptor activator of NF-κB ligand; Runx2, runt-related transcription factor 2.