Figure 5

Expression of chemokines CCL2 , CCL3 , CCL4 , and IL-1β , Fas and Fas ligand ( FasL ) genes in primary synovial fluid (SF)-derived osteoblastogenic cultures. Cultures were derived from children with oligoarticular juvenile idiopathic arthritis (oJIA, n = 12) or polyarticular JIA (pJIA, n = 4) and gene expression analyzed at culture days 14 and 21 (median and interquartile range (IQR), *P < 0.05 vs. oJIA, Mann-Whitney test). mRNA expression was calculated according to the standard curve for each gene expression in the calibrator sample (cDNA from osteoblast cultures) and normalized to the mRNA quantity for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (endogenous control).