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Table 2 Osteoblast gene expression in primary and fourth passage (P4) SF-derived osteoblast cultures from patients with juvenile idiopathic arthritis*

From: Osteoblastogenesis from synovial fluid-derived cells is related to the type and severity of juvenile idiopathic arthritis

  Primary cultures
Gene oJIA (n = 12) pJIA (n = 4)
  Day 14 Day 21 Day 14 Day 21
Runx2 0.02 (0.01-0.07) 0.05 (0.02-0.11) 0.00 (0.00-0.01) 0.02 (0.00-0.05)
P (vs. pJIA) 0.02 0.18   
P (vs. day 21) 0.20   0.34  
OPG 1.63 (0.39-5.65) 13.99 (8.22-21.82) 0.68 (0.40-1.48) 1.52 (0.50-5.68)
P (vs. pJIA) 0.47 0.03   
P (vs. day 21) 0.001   0.49  
RANKL 0.08 (0.00-0.16) 0.18 (0.04-0.50) 0.12 (0.03-0.20) 0.16 (0.06-0.20)
P (vs. pJIA) 0.54 0.90   
P (vs. day 21) 0.42   0.89  
  P4 cultures
  oJIA (n = 7) pJIA (n = 4)
  Day 10 Day 14 Day 10 Day 14
Runx2 0.04 (0.02-0.08) 0.03 (0.02-0.05) 0.02 (0.01-0.02) 0.02 (0.01-0.03)
P (vs. pJIA) 0.02 0.32   
P (vs. day14) 0.46   0.20  
OPG 0.07 (0.04-0.08) 0.12 (0.08-0.20) 0.04 (0.01-0.06) 0.04 (0.01-0.06)
P (vs. pJIA) 0.23 0.01   
P (vs. day14) 0.04   0.89  
RANKL 0.01 (0.01-0.03) 0.01 (0.01-0.04) 0.00 (0.00-0.00) 0.01 (0.00-0.01)
P (vs. pJIA) 0.04 0.23   
P (vs. day14) 0.53   0.06  
  1. *Values are median (interquartile range), statistical differences were calculated using the Mann-Whitney test. Patients were subdivided into the oligoarticular (oJIA) or polyarticular (pJIA) group according to the International League of Associations for Rheumatology (ILAR) criteria [3]. Day, day of osteoblast culture; OPG, osteoprotegerin gene; RANKL, receptor activator of NF-κB ligand gene; Runx2, runt-related transcription factor 2 gene.