Arthritis Research & Therapy

Table 2 Osteoblast gene expression in primary and fourth passage (P4) SF-derived osteoblast cultures from patients with juvenile idiopathic arthritis*

From: Osteoblastogenesis from synovial fluid-derived cells is related to the type and severity of juvenile idiopathic arthritis

	Primary cultures
Gene	oJIA (n = 12)		pJIA (n = 4)
	Day 14	Day 21	Day 14	Day 21
Runx2	0.02 (0.01-0.07)	0.05 (0.02-0.11)	0.00 (0.00-0.01)	0.02 (0.00-0.05)
P (vs. pJIA)	0.02	0.18
P (vs. day 21)	0.20		0.34
OPG	1.63 (0.39-5.65)	13.99 (8.22-21.82)	0.68 (0.40-1.48)	1.52 (0.50-5.68)
P (vs. pJIA)	0.47	0.03
P (vs. day 21)	0.001		0.49
RANKL	0.08 (0.00-0.16)	0.18 (0.04-0.50)	0.12 (0.03-0.20)	0.16 (0.06-0.20)
P (vs. pJIA)	0.54	0.90
P (vs. day 21)	0.42		0.89
	P4 cultures
	oJIA (n = 7)		pJIA (n = 4)
	Day 10	Day 14	Day 10	Day 14
Runx2	0.04 (0.02-0.08)	0.03 (0.02-0.05)	0.02 (0.01-0.02)	0.02 (0.01-0.03)
P (vs. pJIA)	0.02	0.32
P (vs. day14)	0.46		0.20
OPG	0.07 (0.04-0.08)	0.12 (0.08-0.20)	0.04 (0.01-0.06)	0.04 (0.01-0.06)
P (vs. pJIA)	0.23	0.01
P (vs. day14)	0.04		0.89
RANKL	0.01 (0.01-0.03)	0.01 (0.01-0.04)	0.00 (0.00-0.00)	0.01 (0.00-0.01)
P (vs. pJIA)	0.04	0.23
P (vs. day14)	0.53		0.06

*Values are median (interquartile range), statistical differences were calculated using the Mann-Whitney test. Patients were subdivided into the oligoarticular (oJIA) or polyarticular (pJIA) group according to the International League of Associations for Rheumatology (ILAR) criteria [3]. Day, day of osteoblast culture; OPG, osteoprotegerin gene; RANKL, receptor activator of NF-κB ligand gene; Runx2, runt-related transcription factor 2 gene.

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