Hyaluronan injection in murine osteoarthritis prevents TGFbeta 1-induced synovial neovascularization and fibrosis and maintains articular cartilage integrity by a CD44-dependent mechanism

Table 3 QPCR of chondrogenic and fibrogenic gene expression in meniscus/synovium

	acan	Fold²	col2a	Fold²	col10a	Fold²	col1a	Fold²	col3a	Fold²	col5a	Fold²
	ΔCT¹		ΔCT¹		ΔCT¹		ΔCT¹		ΔCT¹		ΔCT¹
Naive	3.28	1.00	3.73	1.00	5.50	1.00	-0.21	1.00	0.05	1.00	3.46	1.00
Acute	-0.89	17.5	-1.59	39.9	1.92	11.9	-6.89	102	-0.13	1.13	1.43	4.08
TTR	0.79	5.61	-1.41	35.3	1.35	17.7	-4.90	25.8	-3.66	13.1	-1.40	29.0
TTR+HA	2.99	1.22	2.63	2.10	4.76	1.67	-1.45	2.43	-1.11	2.23	2.12	2.53
TTR+SA	1.10	4.53	-1.25	31.5	1.46	16.4	-6.10	59.3	-2.68	6.63	-0.98	21.7

¹relative to GAPDH, mean (SD) ΔCT of 2 pools with 8 mice per pool for Naïve and 2 pools with 12 mice per pool for all 4 treatment groups. Values represent the average of the two pools, with the difference between duplicates being < 20% of average pool value.
²Fold Changes are relative to Naive

ISSN: 1478-6362