Figure 1
From: Autoantibodies directed to novel components of the PM/Scl complex, the human exosome
Anti exosome ELISA data obtained with sera from anti-PM/Scl-positive patients. Sera from patients with IIM, scleroderma, and myositis/scleroderma overlap were analyzed by ELISA for reactivity with six novel components of the human exosome (hRrp4p, hRrp40p, hRrp41p, hRrp42p, hRrp46p, and hCsl4p). A pool of 10 normal human sera was run on each ELISA plate to determine the absorbance cutoff value, which was set at three times the absorbance of this negative control. The figure shows all positive ELISA signals corrected for background by subtracting the cutoff value for each individual ELISA plate. The corrected ELISA signals of anti-PM/Scl-positive IIM sera (open squares), scleroderma sera (circles) and myositis/scleroderma overlap syndrome sera (filled squares) are indicated. OD450 = optical density at λ450 nm.