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Table 1 Source and characteristics of fibroblasts used for mRNA quantification

From: Cytokine mRNA and protein expression in primary-culture and repeated-passage synovial fibroblasts from patients with rheumatoid arthritis

Source of isolated cells Number of patients Method of cell isolation Median no. of passages (range)
Rheumatoid arthritis (synovial membrane)    
   Primary culture 5 Collagenase digest 0
   Passage ≥ 5 7 Tissue outgrowth 9 (5–14)
Foreskin (normal skin)    
   Primary culture 6 Collagenase digest 0
   Passage ≥ 5 6 Tissue outgrowth 5.5 (5–13)