Genetic mismatch affects the immunosuppressive properties of mesenchymal stem cells in vitro and their ability to influence the course of collagen-induced arthritis

Table 1 Bone marrow stromal cells from all three mouse strains had characteristics of mesenchymal stem cells in terms of morphology, differentiation capacity and cell surface markers.

Mouse Strain	DBA/1	FVB	BALB/c
MHC H2 Haplotype	H2^q	H2^q	H2^d
Morphology	Fibroblastic	Fibroblastic	Fibroblastic
Adipogenesis	Positive	Positive	Positive
Osteogenesis^a (μg Ca/well)	6.9 ± 1.6	48.0 ± 0.9	11.9 ± 0.2
Chondrogenesis^a (μg GAG/pellet)	5.6 ± 0.3	5.6 ± 0.5	5.6 ± 0.3
CD73^b	64.8	97	17.2
CD105^b	82	49.5	97.9
Sca 1^b	89.9	67.5	53
CD29	99.2	99.4	96.2
CD44	95.1	98.7	99.7
CD45^b	0.6	4	0.6
CD34^b	0.4	0.4	0.8
CD31^b	0.4	0.5	1.3

^aData represents mean ± standard deviation of triplicate assays; ^b % positive cells; GAG, glycosaminoglycan; MHC, major histocompatibility complex.

ISSN: 1478-6362