Table 2 Primary and secondary antibodies for immunofluorescence staining
From: Angiotensin II induces skin fibrosis: a novel mouse model of dermal fibrosis
| Â | Primary antibodies | Secondary antibodies | ||
|---|---|---|---|---|
Myofibroblasts | Rabbit anti-mouse αSMA Ab (Novus Biologicals, Littleton, CO); 1:100 | Alexa fluor 488 donkey anti-rabbit IgG (Invitrogen, Grand Island, NY); 1:1,000 | ||
Fibrocytes | Rat anti-mouse CD45 Ab (BD Pharmingen, San Diego, CA); 1:50 | Rabbit anti-mouse FSP1 Ab (Abcam, Cambridge, MA); 1:100 | Alexa fluor 594 donkey anti-rat IgG (Invitrogen); 1:1,000 | Alexa fluor 488 donkey anti-rabbit IgG (Invitrogen); 1:1,000 |
EndoMT | Goat anti-mouse VE-cadherin Ab (Santa Cruz Biotechnology, Santa Cruz, CA); 1:300 | Rabbit anti-mouse FSP1 Ab (Abcam); 1:100 | Alexa fluor 488 donkey anti-goat IgG (Invitrogen); 1:1,000 | Alexa fluor 594 donkey anti-rabbit IgG (Invitrogen); 1:1,000 |