Table 1 Forward and reverse primer sequences used for real-time PCR
Species | Gene | Forward sequence | Reverse sequence |
|---|---|---|---|
Human | ADAMTS-4 | 5'-CCCCAGACCCCGAAGAGCCA-3' | 5'-CCCGCTGCCAGGCACAGAAG-3' |
Human | ADAMTS-5 | 5'-GGCCGTGGTGAAGGTGGTGG-3' | 5'-GCTGCGTGGAGGCCATCGTC-3' |
Human | ACAN | 5'-ACCAGACGGGCCTCCCAGAC-3' | 5'-TGGCTCTGCCCCAGAGGGAC-3' |
Human | COL21A | 5'-TGAGGGCGCGGTAGAGACCC-3' | 5'-TGCACACAGCTGCCAGCCTC-3' |
Human | IL-1Ra (pair 1)* | 5'-CCCGTGAAGGAGAGCCCTTCATTTG-3' | 5'-ACTTTCACCATCATTTCACAAATGCAG-3' |
Human | IL-1Ra (pair 2)** | 5'-TGTTCCATTCAGAGACGATCTGCCG-3' | 5'-GAGCATGAGGCTCAATGGGTACC-3' |
Human | MMP-13 | 5'-ATGCGGGGTTCCTGATGTGG-3' | 5'-GGCCCAGGAGGAAAAGCATG-3' |
Murine | Acan | 5'-CAGTGCGATGCAGGCTGGCT-3' | 5'-CCTCCGGCACTCGTTGGCTG-3' |
Murine | Col2a1 | 5'-CACACTGGTAAGTGGGGCAAGACCG-3' | 5'-GGATTGTGTTGTTTCAGGGTTCGGG-3' |
Murine | IL-1Ra | 5'-ACCCATGGCTTCAGAGGCAGC-3' | 5'-GCCCCCGTGGATGCCCAAG-3' |