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Table 1 Forward and reverse primer sequences used for real-time PCR

From: Matrilin-3 Induction of IL-1 receptor antagonist Is required for up-regulating collagen II and aggrecan and down-regulating ADAMTS-5 gene expression

Species Gene Forward sequence Reverse sequence
Human ADAMTS-4 5'-CCCCAGACCCCGAAGAGCCA-3' 5'-CCCGCTGCCAGGCACAGAAG-3'
Human ADAMTS-5 5'-GGCCGTGGTGAAGGTGGTGG-3' 5'-GCTGCGTGGAGGCCATCGTC-3'
Human ACAN 5'-ACCAGACGGGCCTCCCAGAC-3' 5'-TGGCTCTGCCCCAGAGGGAC-3'
Human COL21A 5'-TGAGGGCGCGGTAGAGACCC-3' 5'-TGCACACAGCTGCCAGCCTC-3'
Human IL-1Ra (pair 1)* 5'-CCCGTGAAGGAGAGCCCTTCATTTG-3' 5'-ACTTTCACCATCATTTCACAAATGCAG-3'
Human IL-1Ra (pair 2)** 5'-TGTTCCATTCAGAGACGATCTGCCG-3' 5'-GAGCATGAGGCTCAATGGGTACC-3'
Human MMP-13 5'-ATGCGGGGTTCCTGATGTGG-3' 5'-GGCCCAGGAGGAAAAGCATG-3'
Murine Acan 5'-CAGTGCGATGCAGGCTGGCT-3' 5'-CCTCCGGCACTCGTTGGCTG-3'
Murine Col2a1 5'-CACACTGGTAAGTGGGGCAAGACCG-3' 5'-GGATTGTGTTGTTTCAGGGTTCGGG-3'
Murine IL-1Ra 5'-ACCCATGGCTTCAGAGGCAGC-3' 5'-GCCCCCGTGGATGCCCAAG-3'
  1. *Primer pair used for IL-1Ra expression analysis in C28/I2 immortalized human chondrocytes and primary human chondrocytes for Figure 1A, 1C and Additional file 1. **Primer pair used for IL-1Ra expression analysis in primary human chondrocytes for Figure 4A and 4B. ACAN, aggrecan; COL2A1, type II collagen; IL-1Ra, interleukin-1 receptor antagonist; MMP-13, matrix metalloproteinase-13.