Validation of a multiplex chip-based assay for the detection of autoantibodies against citrullinated peptides

Table 1 Citrullinated peptides in the ACPA microarray

Peptide	Protein	Amino acids	Amino acid sequence	Reference
CEP-1/Eno5-21	α-Enolase	5-21	CKIHA(cit)EIFDS(cit)GNPTVEC (cyclic)	[16]
Vim60-75	Vimentin	60-75	VYAT(cit)SSAV(cit)L(cit)SSVP	[37]
Vim2-17	Vimentin	2-17	ST(cit)SVSSSSY(cit)(cit)MFGG	[33]
CCP-1/Fil307-324	Filaggrin	307-324	SHQEST(cit)GRSRGRSGRSGS (cyclic)	[3, 32]
Fibβ36-52	Fibrinogen β-chain	36-52	NEEGFFSA(cit)GHRPLDKK	[37]
Fibβ563-583	Fibrinogen β-chain	563-583	HHPGIAEFPS(cit)GKSSSYSKQF	[13]
Fibβ580-600	Fibrinogen β-chain	580-600	SKQFTSSTSYN(cit)GDSTFESKS	^c
Fibβ62-81a^a	Fibrinogen β-chain	62-81	APPPISGGGY(cit)ARPAKAAAT	[13]
Fibβ62-81b^b	Fibrinogen β-chain	62-81	APPPISGGGYRA(cit)PAKAAAT	[13]
Fibβ60-74	Fibrinogen β-chain	60-74	(cit)PAPPPISGGGY(cit)A(cit)	[12, 31]
Fibα621-635	Fibrinogen α-chain	621-635	(cit)GHAKS(cit)PV(cit)GIHTS	[12, 31]
citC1/CII359-369	Collagen type II	359-369	(GPO)5-GA(cit)GLTG(cit)PGDA(GPO)2-GKKYG	[20, 28]

The arginine-containing control peptides have identical sequences, except that they contain arginine residues instead of citrulline (Cit, citrulline). Peptides are designated by their abbreviated protein names (Eno, α-enolase; Vim, vimentin; Fib, fibrinogen; CII, type II collagen) followed by the positions of the first and last amino acid. For three peptides, earlier given specific names (CEP-1, CCP-1, citC1), these names are given in parallel. ^aCitrullinated at position 72. Uses the same control peptide containing two arginines as for Fibβ62-81b. ^bCitrullinated at position 74. Uses the same control peptide containing two arginines as for Fibβ62-81a. ^cThe citrullinated epitope in this peptide has been found to be citrullinated in RA synovial tissue (Per-Johan Jakobsson, personal communication).

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