Figure 3

Cardiac mitochondrial NADP+-dependent isocitrate dehydrogenase (mNADP-ICDH) activity (A), protein (B), and mRNA (C). Total proteins and RNA were extracted from left ventricular (LV) tissues of control and adjuvant-induced arthritis (AIA) rats and processed to enzymatic assay (A), Western blotting (B) or real-time PCR (C), as described under Materials and Methods. (D) Cardiac 4-hydroxynonenal (HNE)/NADP-ICDH adducts were identified in total proteins by immunoprecipitation as described in Material and Methods. Values are the mean ± SEM of three experiments. **P < 0.01 (CTL versus AIA), ^#P < 0.05, ^&P < 0.01 (AIA versus AIA+Ram) by one-way ANOVA. CTL, control; Ram, ramipril.