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Table 1 Effects of zidovudine on soleus muscle histology, serum parameters, mtDNA, mitochondrial function, and muscle fiber-type characteristics

From: Muscle-fiber transdifferentiation in an experimental model of respiratory chain myopathy

Complete soleus muscle Control (n= 10) Zidovudine (n= 10) P value
Soleus muscle fiber diameter (median μm) 58.3 (IQR 54.2, 62.3) 41.1 (IQR 37.5, 47.1) <0.001
Serum creatine kinase (U/L) 724 ± 480 548 ± 257 0.3
Serum creatinine (mg/L) 3.6 ± 0.34 3.1 ± 0.11 <0.001
Serum glucose (mg/L) 1,760 ± 100 1,680 ± 220 0.055
Serum resting lactate (mM) 4.5 ± 1.8 5.0 ± 1.6 0.9
Serum alanine aminotransferase (U/L) 66 ± 8 56 ± 7 0.1
Serum aspartate aminotransferase (U/L) 125 ± 35 101 ± 13 0.09
mtDNA copy number (mean copies/myonucleus) 559 ± 46 399 ± 78 <0.001
NADH DH (μmoles min-1 g muscle protein-1 ) 684 ± 254 474 ± 236 0.042
COX (μmoles min-1 g muscle protein-1 ) 28 ± 8 18 ± 9 0.026
SDH (μmoles min-1 g muscle protein-1 ) 36 ± 6 35 ± 10 0.7
NADH DH/SDH-ratio (% of control mean) 100 ± 28 72 ± 20 0.027
COX/SDH-ratio (% of control mean) 100 ± 27 67 ± 26 0.016
Single-fiber soleus muscle Control Zidovudine p-value
Fiber type Fast Slow Fast Slow Fast Slow
Fiber-type proportion (% of all fibers) 5.2 ± 3.0 94.8 ± 3.0 13.3 ± 5.1 86.7 ± 5.1 <0.001 <0.001
Fiber diameter (mean μm) 54.8 ± 5.8 65.5 ± 10.6 37.3 ± 9.3 51.8 ± 6.2 <0.001 0.006
mtDNA copy number (mean copies/myonucleus) 617 ± 91 683 ± 94 384 ± 91 206 ± 97 <0.001 <0.001
  1. Values represent group means (±SD); soleus muscle-fiber diameter values are given as median with interquartile range.