CII-specific antibody response at different time points after CIA induction. Blood samples were taken from B10.Q and B10.DR4.Ncf1*/* mice at four time points after rCII immunization (day 15, 35, 50 and 65). Extracted sera were used to assess the levels of anti-CII antibodies, anti-C1, anti-U1 and anti-J1 epitope-specific antibodies. (A) Total anti-CII antibody concentration in sera of B10.DR4.Ncf1*/* mice (number of animals per group: day 15 - n = 22, day 35 - n = 16, day 50 - n = 10, day 65 - n = 5). (B) Total anti-CII antibodies concentration in B10.Q mice (n = 20 per each time point). (C) Anti-C1, anti-U1 and anti-J1 antibody levels, measured in B10.DR4.Ncf1*/* mouse strain (number of animals per group: day 15 - n = 22, day 35 - n = 16, day 50 - n = 10, day 65 - n = 5). (D) Levels of anti-CII epitopes (C1, U1, J1) antibodies in B10.Q mice (n = 20 per each time point). Results indicate mean ± SE. CIA, collagen-induced arthritis; CII, collagen type II.