Supplementary Figure 1

Quantitation of functional viral titer following time course optimization. Viral supernatant was centrifuged at 6000 g for the time periods indicated. The viral pellet was resuspended in a thirtieth of the original volume. The viral titer of the post-centrifugation supernatant (solid bars) and the resuspended viral pellet (open bars) were measured on NIH 3T3 cells by the FACS-based limiting dilution expression assay. Data are representative of three similar experiments.