Analysis of IL-36R, IL-36γ and IL-36Ra mRNA expression in normal and inflamed joints during collagen-induced arthritis (CIA). IL-36 receptor (IL-36 R) (A), IL-36γ (B), IL-36R antagonist (IL-36Ra) (C), IL-1RI (D), IL-1β (E) and IL-1Ra (F) mRNAs levels were determined by RT-qPCR in normal (clinical score = 0) or inflamed (low clinical scores: 0.5 to 1.5; high clinical scores: 2.0 to 3.0) ankles of type II collagen (CII)-immunized DBA-1 mice in the first nine days after the appearance of clinical symptoms (early arthritis). (A-F) mRNA expression was normalized to the amount of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA. Each data point represents a single mouse; horizontal lines show the mean. *P < 0.05 versus score 0, as assessed by analysis of variance, followed by unpaired two-tailed Student's t-test.