Figure 4

Exogenous human granulocyte-colony stimulation factor (huG-CSF) restores autoantibody (autoAb) production in B6. Sle2c2 mice after induction of chronic graft vs host disease (cGVHD). (A) huG-CSF-treatment induced anti-dsDNA IgG in B6.Sle2c2 mice in a dose-dependent manner (left), but had little effect in B6 mice (right). Five mice per strain per treatment were used. (B) Anti-dsDNA and anti-chromatin IgG found at d21 in the combined 120 × 1 and 12 × 2 cohorts. (C) Representative ANA IgG staining of Hep-2 cells incubated with sera collected at 21 d in each of the four treatment groups in the 120 × 1 and 12 × 2 cohorts and average staining intensity of 10 to 20 randomly selected cells per sample. Means and standard error of the mean with the significance value from the t-test for comparison of treated mice and controls (dex) are shown for each strain for a given time point in A, and from Dunnett's multiple comparison test between groups as indicated in B and C (*P < 0.05; **P < 0.01; ***P < 0.001).