Figure 4
From: Fragmentation of Golgi complex and Golgi autoantigens during apoptosis and necrosis
Apoptotic cleavage of Golgi autoantigens is caspase dependent. Benzylocarbonyl-Val-Ala-Asp-fluromethylketone (zVAD-fmk) (100 μM) was added to Jurkat cells 1 hour prior to addition of staurosporine (STS). Cells were then exposed to STS for 4 hours prior to harvest for lysate preparation. Representative blots of lysates from untreated control, 1 μM STS treatment without zVAD-fmk, and 1 μM STS treatment with zVAD-fmk are shown. Intact protein and cleavage fragments are indicated. Numbers to left of each blot represent the relative molecular weight (kDa). g160, golgin-160; g97, golgin-97; gm130, golgin-95; PARP, polyADP-ribose polymerase. See Figure 3 for explanation of a–e.