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Table 1 Phenotypic characterization of adipose-derived stem cells through immunophenotype and RT-PCR

From: Analyses of chondrogenic induction of adipose mesenchymal stem cells by combined co-stimulation mediated by adenoviral gene transfer

Analyzed marker

Immunophenotypea

RT-PCRb

Positivec

  

   CD73

NT

++

   CD90

NT

++

   CD105

NT

++

   CD166

NT

++

   CD271

++

++

   MSCA

++

NT

Negativec

  

   CD14

NT

+

   CD34

NT

-

   CD45

+

-

   CD117

NT

-

GAPDHd

NT

++

  1. aNT, not tested; +, positive (≤50%); ++, positive (≥85%). bNT, not tested; -, negative; +, positive (faint band); ++, positive (intense band). cExpected result of surface antigen expression on mesenchymal stem cells according to the literature [22, 23]. dHousekeeping gene.