Supplementary Figure 1
The strategy of our experiments. SF from one patient with RA (passage6) were cultured in 75 cm2 flasks until reaching confluence. Then, these cells were collected and seeded in 12-well culture plates (1.0 × 105 cells/well). At 1, 2, 4, 7, 10 and 14 days after seeding, the number of cells was counted by the trypan blue exclusion method. At 4 days, the cells at low density (LD) proliferated exponentially, and at 14 days, the cells at high density (HD) almost ceased to proliferate as a result of contact inhibition. The inserts show the HE staining of SF on chamber slides at 4 and 14 days after seeding, respectively.