Dynamic pressurization induces transition of notochordal cells to a mature phenotype while retaining production of important patterning ligands from development

Table 1 Proteomic analysis of conditioned media samples from Daily dynamic and unloaded Control groups demonstrating significant peptide differences between groups >2

Protein	Control		Daily load		Peptide difference	P value	Function
	Mean number of peptides	SD	Mean number of peptides	SD
Vimentin	4	1.73	0	0	-4.00	0.02	Cytoskeletal protein: responsive to mechanical load [23, 40, 55]
Heat shock protein 70 kDa	3.7	0.6	1	1.6	-2.7	0.02	Notochordal cells have lower levels than small nucleus pulposus cells][47]
Phosphoglycerate mutase-1	2.3	1.2	0.0	0	-2.3	0.02	Glycolytic pathway [56]
α₁-Antitrypsin	3.3	1.2	0.7	0.6	-2.7	0.05	Inhibits serine proteases and can bind aggrecanases [52]
Glucose-6-phosphate isomerase	15.66	6.50	6.33	1.53	-9.33	0.07	Glycolytic pathway [57]

Proteomics identified five proteins that were differentially expressed between loaded and daily loaded groups. SD, standard deviation.

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