Glycophenotyping of osteoarthritic cartilage and chondrocytes by RT-qPCR, mass spectrometry, histochemistry with plant/human lectins and lectin localization with a glycoprotein

Toegel, Stefan; Bieder, Daniela; André, Sabine; Altmann, Friedrich; Walzer, Sonja M; Kaltner, Herbert; Hofstaetter, Jochen G; Windhager, Reinhard; Gabius, Hans-Joachim

doi:10.1186/ar4330

Table 1 Panel of lectins (plant agglutinins and human galectins) used for glycophenotyping of human OA cartilage

From: Glycophenotyping of osteoarthritic cartilage and chondrocytes by RT-qPCR, mass spectrometry, histochemistry with plant/human lectins and lectin localization with a glycoprotein

Plant and lectins	Abbreviation	Monosaccharide specificity	Optimized concentration (ng/μl)	Potent oligosaccharide/glycoprotein glycan ligands
Canavalia ensiformis (jack bean) agglutinin	ConA	Man/Glc	0.5	Manα6(Manα3)Manβ4GlcNAcβ4GlcNAc
Pisum sativum (pea) agglutinin	PSA	Man/Glc	0.5	N-glycan binding enhanced by core fucosylation
Phaseolus vulgaris (kidney bean) erythroagglutinin	PHA-E	^a	1	Bisected complex-type N-glycans: Galβ3/4GlcNAcβ2Manα6(GlcNAcβ2-Manα3)(GlcNAcβ4)Manβ4GlcNAcβ4GlcNAc
Phaseolus vulgaris (kidney bean) leukoagglutinin	PHA-L	^a	1	Tetra- and triantennary N-glycans with β6-branching
Viscum album (mistletoe) agglutinin	VAA	Gal	0.5	Galβ3(4)GlcNAc without/with α2,6-sialylation, Galα3(4)Gal, Galβ2(3)Gal, Fucα2Gal
Lycopersicon esculentum (tomato) agglutinin	LEA	^a	2	core and stem regions of high-mannose-type N-glycans, (GlcNAcβ3Galβ4GlcNAcβ3Gal) repeats (LacDiNAc to polyLacNAc)
Maackia amurensis agglutinin-I (leukoagglutinin)	MAA-I	^a	20	Neu5Ac/Gcα3Galβ4GlcNAc/Glc^b, 3′-sulfation instead of sialylation and 9′-O-acetylation tolerated
Sambucus nigra (elderberry) agglutinin	SNA	Gal/GalNAc	0.4	Neu5Ac/Gcα6Gal/GalNAc^c, clustered T_n-antigen, 9′-O-acetylation tolerated
Dolichos biflorus (horse gram) agglutinin	DBA	GalNAc	50	GalNAcα3GalNAcα3Galβ4Galβ4Glc, clustered T_n-antigen, histo-blood group A- tetrasaccharide, β-linked GalNAc in Sd^a antigen
Arachis hypogaea (peanut) agglutinin	PNA	Gal	0.7	Galβ3GalNAcα/β
Artocarpus integrifolia (jack fruit) agglutinin	Jacalin (JAC)	Gal/GalNAc	0.7	Galβ3GalNAcα, sialylation of T/T_n antigens tolerated
Galectin-1	Gal-1	^d	1	Type I/II disaccharides (α2,3-sialylation/sulfation tolerated), LacNAc repeats (terminal α2,6-sialylation not tolerated), Fucα2Gal, extended core 2/4 structures, multiantennary N-glycans
Galectin-3	Gal-3	^d	1	Type I/II and core 1 disaccharides (α2,3-sialylation/sulfation tolerated), LacNAc repeats (terminal α2,6-sialylation tolerated), GalNAcβ4GlcNAc (LacdiNAc), Galα3Galβ4GlcNAc, histo-blood group ABH epitopes, multiantennary N-glycans/clustered T-antigen

^ano monosaccharide known as ligand; ^bbinding specific for type II LacNAc (Galβ4GlcNAc) core [32]; ^cbinding of type I LacNAc (Galβ3GlcNAc) core preferred, 6′-sulfation of GlcNAc in α2,6-sialylated LacNAc (type I/II) enhances affinity [33]; ^dextension of Gal core to disaccharide (mostly in β-linkage such as Galβ2Gal or Galβ4Glc) required.

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ISSN: 1478-6362

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