Mast cell-induced prostaglandin E
production by human tenocytes is mediated through transforming growth factor beta-1. Tenocytes were stimulated with HMC-1 conditioned media (cond media) with or without indomethacin (2 μM) or A83-01 (2 μM) for 6 hours. Media were removed, cells were washed three times with phosphate-buffered saline, and incubated with a fresh serum-free media for 24 hours. Enzyme-linked immunosorbent assay measured the prostaglandin E2 (PGE2) concentration in tenocyte-conditioned media. Data represents mean ± standard error of the mean of at least three separate experiments. One-way analysis of variance, P <0.001. *Statistically significant Bonferroni post-hoc tests of P <0.01.